Journal:

Article Title: Elucidation of Listeria monocytogenes Contamination Routes in Cold-Smoked Salmon Processing Plants Detected by DNA-Based Typing Methods

doi: 10.1128/AEM.67.6.2586-2595.2001

Figure Lengend Snippet: Adapter and primer oligonucleotides used for AFLP DNA typing of L. monocytogenes from cold-smoked salmon processing plants

Article Snippet: Genomic DNA were extracted from strains of L. monocytogenes by using an Easy-DNA kit (Invitrogen, De Schelp, The Netherlands) according to the manufacturer's instructions.

Techniques: Sequencing

Journal:

Article Title: Elucidation of Listeria monocytogenes Contamination Routes in Cold-Smoked Salmon Processing Plants Detected by DNA-Based Typing Methods

doi: 10.1128/AEM.67.6.2586-2595.2001

Figure Lengend Snippet: Combined dendrogram for 30 L. monocytogenes isolates analyzed by RAPD, AFLP, and PFGE typing. The dendrogram was constructed with GelCompar and Bionumerics (Applied Maths) software by using the Dice correlation and cluster analysis by the unweighted pair group method using arithmetic averages. Percentages of similarity are shown above the dendrogram. The molecular sizes of RAPD, AFLP, and PFGE DNA ranged from 200 to 2,000 bp, from 40 to 500 bp, and from 50 to 400 kb, respectively. The origins of strains are shown in Table ​Table77.

Article Snippet: Genomic DNA were extracted from strains of L. monocytogenes by using an Easy-DNA kit (Invitrogen, De Schelp, The Netherlands) according to the manufacturer's instructions.

Techniques: Construct, Software

Journal: BioTechniques

Article Title: Limitations of methods for measuring the concentration of human genomic DNA and oligonucleotide samples

doi: 10.2144/btn-2017-0102

Figure Lengend Snippet: Absorbance control values of the samples at 1 cm pathlength measured using a high accuracy dual beam spectrophometer at 22°C and 0.8 nm spectral bandwidth. The values in the parentheses are CV (%) of the mean from N = 9 (genomic DNA) and N = 4 (oligoncleotide samples), except for the *SRM 2082 components that are the standard uncertainties calculated as described in the NIST certificate of analysis ( https://www.nist.gov/srm ) calcuated using extensive measurements.

Article Snippet: Human genomic DNA (gDNA) was obtained from Coriell Institute for Medical Research (Camden, NJ).

Techniques: Control

Journal: BioTechniques

Article Title: Limitations of methods for measuring the concentration of human genomic DNA and oligonucleotide samples

doi: 10.2144/btn-2017-0102

Figure Lengend Snippet: Percentage of control values and coefficients of variation (% CV) using microvolume spectrophometers (MV Spec), and a microvolume (MV) plate reader. The values are the average of data acquired over three days as shown in , with the exception of MV Spec C, where the data were acquired in one day. The values in the parentheses are 1 CV (%) and the number (N) of measurements.

Article Snippet: Human genomic DNA (gDNA) was obtained from Coriell Institute for Medical Research (Camden, NJ).

Techniques: Control

Journal: BioTechniques

Article Title: Limitations of methods for measuring the concentration of human genomic DNA and oligonucleotide samples

doi: 10.2144/btn-2017-0102

Figure Lengend Snippet: Mass concentration values of human genomic DNA samples obtained using the different methods. Absorbance values and derived mass values of the human genomic DNA samples from a double-beam (DB) spectrophotometer, microliter volume (MV) spectrophotometers, microvolume (MV) plate reader, fluorescent dye binding, and digital PCR assays.

Article Snippet: Human genomic DNA (gDNA) was obtained from Coriell Institute for Medical Research (Camden, NJ).

Techniques: Concentration Assay, Derivative Assay, Spectrophotometry, Binding Assay, Digital PCR, Lambda DNA Preparation

Journal: BioTechniques

Article Title: Limitations of methods for measuring the concentration of human genomic DNA and oligonucleotide samples

doi: 10.2144/btn-2017-0102

Figure Lengend Snippet: Mass concentration of the human genomic DNA sample calculated from droplet digital PCR data with different assumptions of the droplet size.

Article Snippet: Human genomic DNA (gDNA) was obtained from Coriell Institute for Medical Research (Camden, NJ).

Techniques: Concentration Assay, Digital PCR

Journal:

Article Title: Efficient Approach to Unique Single-Nucleotide Polymorphism Discovery

doi:

Figure Lengend Snippet: The results of scanning sWXD3868 for SNPs by method 1 is shown in A. CEPH parents are 1, 2, 3, 4, and the CEPH population pool is 5 (additional detail about DNAs used are included in Methods). Sequencing was done with the dRhodamine terminators in A. The results for method 2 are shown in B. The CHM1 is sample 6 and the CEPH population pool is sample 5. Sequencing was done with the BigDye terminators in B. (↓) SNP locations. The small blue underhand for the T peak (↓) in B, sample 6 is a common sequencing artifact and was seen in a number of T peaks in this sequencing trace.

Article Snippet: Genomic DNA samples from 4 female CEPH parents (individual 1, CEPH K102-02, Coriell NA04479; individual 2, CEPH K1340-02, Coriell NA07019; individual 3, CEPH K1345-02, Coriell NA07348A; individual 4, CEPH K13294-02, Coriell NA07434 Coriell Institute; 8 chromosomes) and a pooled genomic DNA sample consisting of 40 female and 40 male CEPH parents (120 X chromosomes) were amplified by PCR.

Techniques: Sequencing

Journal:

Article Title: Efficient Approach to Unique Single-Nucleotide Polymorphism Discovery

doi:

Figure Lengend Snippet: The results of scanning sWXD3654 for SNPs by methods 1 and 2 are shown. (Method 1) CEPH parents are 1, 2, 3, and 4 and the CEPH population pool is 5 (additional detail about DNAs used is included in Methods); (method 2) CHM1 is sample 6, and the CEPH population pool is sample 5. The CEPH population pool is shown only once. Sequencing was done with the BigDye terminators. (↓) SNP locations.

Article Snippet: Genomic DNA samples from 4 female CEPH parents (individual 1, CEPH K102-02, Coriell NA04479; individual 2, CEPH K1340-02, Coriell NA07019; individual 3, CEPH K1345-02, Coriell NA07348A; individual 4, CEPH K13294-02, Coriell NA07434 Coriell Institute; 8 chromosomes) and a pooled genomic DNA sample consisting of 40 female and 40 male CEPH parents (120 X chromosomes) were amplified by PCR.

Techniques: Sequencing